Journal of Emerging Trends in Engineering and Applied Sciences: Research Areas

Article Title: PROCESS AND MATHEMATICAL MODEL IN THE FORMULATION OF MICROBIOLOGICAL MEDIA FROM THE EXOSKELETON OF CRUSTACEANS
by Egwari, L. O., and Oniha, M. I

Abstract:
Culture media are very central in the study of microorganisms. Most microbiological media are formulated with beef or soya as protein source. The need for cheaper source of raw material is exploited with the use of exoskeleton of crabs (Callinectes amnicola; shell only) and shrimps (Penaeus notialis; head, shell and appendages). The mathematical model for the formulation derived and the formulated media assessed for nutritional value and ability to support microbial growth. Proximate analyses of infusions from the nutrient sources revealed high contents of minerals especially Na+, K+, Ca2+, Fe2+, Mg2+, SO42-, NO3-, PO42- and traces of glucose. Fats and proteins were higher in crab shell (25.39%, 12.36%) and shrimp exoskeleton (3.39%, 18.48%) respectively. Infusions from these nutrient sources separately or in combination supported good growth of bacteria, moulds and yeasts either as broth or as agar media. The pH changes as a result of microbial growth were minimal thus occluding the need for buffering. The diversity of microorganisms that grew on the media satisfies their requirement as general purpose growth media.

Keywords: crustaceans, exoskeleton, mathematical model, culture media, microorganisms

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