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Journal of Emerging Trends in Engineering and Applied Sciences (JETEAS)
ISSN:2141-7016
| Abstract: This study explored the isolation and characterization of tannase producing bacteria from cowpea (Vigna unguiculata) seeds collected in Epe, Lagos State, Nigeria. Sixteen pure bacterial isolates, including species of Staphylococcus, Micrococcus, Pseudomonas, Enterococcus, and Bacillus, were recovered and biochemically identified. Primary screening using tannic acid agar revealed six tanninolytic isolates, with three (DVN?5, DVN?8, DVN?17A) demonstrating the largest zones of hydrolysis (up to 21?mm). Submerged fermentation over 5 days led to crude enzyme yields, with the top isolate (A1) exhibiting the highest tannase activity (32.194?U/mL). The enzyme was optimal at 60?°C, pH?9.0, and 0.8% tannic acid concentration, with magnesium (Mg²? at 1?mM) significantly enhancing activity. The study concludes that cowpea harbors diverse tanninolytic bacteria suitable for industrial tannase production under alkaline and thermophilic conditions.yme. |
| Keywords: Tannase, Tannic Acid, Cowpea (Vigna Unguiculata), Tannin-Degrading Bacteria, Enzyme Activity, Bacillus Spp., Submerged Fermentation, Alkaline Conditions, Thermostable Enzyme. |
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