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Journal of Emerging Trends in Engineering and Applied Sciences (JETEAS)

ISSN:2141-7016

Article Title: Role Of Glutamate Zerovalent Iron Nanoparticles On Amelioration Of Heavy Metal Stress Tolerance In Maize (Zea Mays L.)
by ?Sami Ullah, Saleha Saeed, Fatima Bibi and Rehman Ullah

Abstract:
The present work was aimed to determine the effect of IAA and glutamate zerovalent iron nanoparticles (Glu-ZVFeNPs) on the physiological mechanism of heavy metal (Pb+2) stress tolerance in the selected maize variety (Pahari white) under induced heavy metal stress of 4ppm and 8ppm at vegetative stage. Seeds of Maize variety (Pahari white) collected from CCRI, PersabaqNowshera, were sown in earther pots (18cm lower inside diameter, 18cm upper inner diameter, 20cm height and 2cm thickness) filled with 2kg of air dried soil and silt (2:1) having EC3.09-5.12, pH 6.8-7.3 and moisture content 4-15% in triplicates in the green house of the Department of Botany, University of Peshawar in maize growing season, 2017. SEM results showed spherical shape of nanoparticles which are uniformly dispersed as observed, coarse surfaces ranges from 45–167nm indicating a high surface area suitable for adsorption properties. EDX analysis showed elemental composition including C, N, Ca+2, Si, Cl- and both the Fe along with O at 0.6 KeV and Zerovalent Iron (Fe0) at 6.5 and 7.1 KeV. TGA/DTA showed the endothermic major decline in mass at 200oC to 400oC and the exothermic major peek formed at 300oC to 400oC. At low concentration of lead (4ppm) the growth responses have been reduced indicating from the minimum amplitude of chlorophyll “a”, chlorophyll “b” and POD content, whereas; this adverse effect has been reduced by application of Glu-ZVFeNPs as separate treatment as compared to the foliar application of IAA alone and in combination with Glu-ZVFeNPs. High concentration of lead (8ppm) enhanced the values of osmolities, protein and SOD whereas the maximum amplitude of proline reduced by the application of Glu-ZVFeNPsas separate treatment indicating that the plant behaves normal with nanoparticle at heavy metal stress of 8ppm.
Keywords: Glu-ZvFe Nanoparticle, Heavy Metal Stress, Proline, Antioxidant Enzymes, TG/DTA
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